By "biosimilar" they mean the lots of the original EUA vaxxes, that are now de-authorized. The Pfizer lots shown are older lots, exp 2022. I will write a more in depth analysis on this. It seems like the FDA is scrambling to get ahead of this issue as they are afraid the investigation will begin under the new admin.
Let's hope Kennedy knows about the messy mess they did with the LNPs and the real damage potential using this crappy tech. Scared about 2035 - 2040. This will be the time were we really can see the exponential and cumulative effects.
Regarding "...afraid the investigation will begin...."
It seems that some things that potentially undermine this paper also reveal reckless assumptions and deceptions by CDC. One step back, 2 steps forward.
As you suggest Siguna Meulere summed it up perfectly. You can find whatever you want to if you look through the right lens to do so - and it seems design you process to facilitate this. Can only leave one outcome - you get what you want.
BIG problem I see in this discussion is it is based upon the belief, not proven in my view, that a pathogenic agent given the name SARS-Cov-2 actually exists and mutational variants can therefore also exist. AND that any one of these may infect a healthy individual.
ALL the rest is pure conjecture, supposition and narrative construction towards the creation of an apparently plausible explanation. Before building and decorating a tree that can stand on its own, first ensure it is well rooted and can support the upper structure you want to create.
Seems like it would be much easier and cheaper just to take quercetin, zinc, vit C, and get some sunshine daily. And never drink crap from the tap or get jabbed! I have not had a cold for 15 years or flu for 20 years and have not been jabbed since 2009.
From my perspective, which is not that of a scientist, this science is full of holes that shouldn't have been left there by the supervising FDA scientists. Was this done intentionally? It seems that the FDA's friends at Pfizer and Moderna can simply say it wasn't their stuff or it was expired and walk away from any responsibility or need to answer any further questions in the public mind. On the positive side, I think the professional scientists involved have been challenged by some high school kids, and they had better answer that challenge and prove them and their methods and theories were wrong, which i don't think they can do. So expect ad hominem attacks. Also, were the FDA scientists may have been trying to say something through the high schoolers without saying it themselves? Heh heh.:-)
This all seems like a bit of a red herring to divert attention from the fact that the medical industrial complex continues to perform horrendous experimentation on humanity.
OK. So was the paper valid, partially valid, invalid? Does the study need to be redone by others to see what they find? I think that would be a good approach.
That's the benefit of Pharma studies with 29,000 subjects- nobody can afford to replicate them. cf "Jupiter Study" for rosuvastatin with the conclusion that every adult male is at risk so should be treated.
The pcDNA3.1(+) is an old skool mammalian expression vector Maria. Its dual purpose becuase it can be used to express a coding sequence in mammalian cells, or transcribe + stranded RNA (which could be a RNA riboprobe, or an mRNA if the RNA is capped and polyadenylated). It was sold by Invitrogen who were later gobbled up by ThermoFisher. The orientation of the multiple cloning site (MCS: a short sequence with useful restriction enzyme sites where you can clone the coding region for the thing you want to express under the control of the strong CMV promoter/enhancer) comes in + or - orientations. In this case it just means that the order of the sites is reversed in - versus +.
The SV40 early promoter/origin or replication has 2 main functions - to drive expression of the neomycin/kanamycin resistance gene to enable selection of transfected DNA (usually integrated as a stable cell line) in cells with neomycin (G418) - as in the Pfizer vector. SV40 ori also allows amplification/maintenance of the plasmid in mammalian cells that carry SV40 large T antigen, such as HEK293T - which gives higher expression of whatever you clone in the MCS.
The ampicillin gene has a bacterial promoter only and is used for selection in E.coli, and replicate the plasmid to high copy number using the pMB1 E.coli origin of replication.
They would have grown the plasmid in E.coli, made a plasmid DNA prep, then linearized the plasmid with an enzyme site that cuts downstream of the sequence they wanted to make mRNA from - then transcribed the + strand as RNA using T7 polymerase.
pMB1 origin (there are others) is needed to replicate the plasmid in E.coli. The Amp promoter/resistance gene (encoding beta-lactamase enzyme) enables selection and maintenance of the plasmid.
They refer in the method to in vitro translation of the plasmid but don't explain how they did it and if they used modified nucleotide or not. I suspect they were give the mRNA and just did the lipid encapsulation using the Cayman kit.
The " bastard class " are still guilty and still continue to cover up , divert , or look away ......all the overwhelming evidence by all the experts just highlights the most important fact .....there was NEVER any informed consent and the public were conspired against .
I applaud all those trying to prove that point .....🤷♂️🙏
Interesting Maria, that you found the 2023 FDA Laboratory award involving 3 named scientists, including Tony T Wang. He has a Phd in microbiology and works at FDA dept of CBER (Center for Biologics Evaluation and Research) which is located at the FDA’s White Oak Campus in Silver Spring, MD, just outside of Washington, DC.
The 3 students (Tyler Wang, Alex Kim & Kevin Kim) from Centerville High School Virginia, won a Science Fair special award in March 2024 for their project (3rd place Certificate $50 split) under "Association for Diagnostics and Laboratory Medicine (ADLM)"
Thanks for the update on this article Dr. Jessica Rose 🌹 this is very confusing to me. My first assumption on reading the first post of this was that they got samples of Moderna and Pfizer and they found in it high DNA contamination this muddies the waters of what this paper really does show in my view ?
Regarding why FDA researchers might be developing a Live Attenuated Vaccine delivered intranasally:
1- Intranasal delivery will lead to mucosal immunity, whereas intramuscular injection leads to very poor mucosal immunity.
2- Omicron is nature's attenuated vaccine but it was first identified in Nov 2021 and had yet to demonstrate how benign it was. The Liu and colleagues paper was received May 10 2022. So at the time, it would've made sense to develop a LAV.
Akiko Iwasaki was trying to get funding for her own intranasal vaccine.
It looks like the LAV is developed through genetic engineering, and not through traditional methods (serial passage through eggs, getting viruses from non-human species, etc.). Operation Warp Speed was very biased towards high-technology approaches and away from low-tech approaches like Sinovac, traditional LAVs, etc. The high-tech approach tends to have patentable components which can generate money for big pharma.
By "biosimilar" they mean the lots of the original EUA vaxxes, that are now de-authorized. The Pfizer lots shown are older lots, exp 2022. I will write a more in depth analysis on this. It seems like the FDA is scrambling to get ahead of this issue as they are afraid the investigation will begin under the new admin.
Yes that’s what I surmised re biosimilars. Just confusing language.
Let's hope Kennedy knows about the messy mess they did with the LNPs and the real damage potential using this crappy tech. Scared about 2035 - 2040. This will be the time were we really can see the exponential and cumulative effects.
Regarding "...afraid the investigation will begin...."
It seems that some things that potentially undermine this paper also reveal reckless assumptions and deceptions by CDC. One step back, 2 steps forward.
Thank you for the clarification, Sasha!
I hope you might post on this investigation at some time.
It's interesting in VAERS lot GL2042 is listed as BOTH mono- and bi-valent
As you suggest Siguna Meulere summed it up perfectly. You can find whatever you want to if you look through the right lens to do so - and it seems design you process to facilitate this. Can only leave one outcome - you get what you want.
BIG problem I see in this discussion is it is based upon the belief, not proven in my view, that a pathogenic agent given the name SARS-Cov-2 actually exists and mutational variants can therefore also exist. AND that any one of these may infect a healthy individual.
ALL the rest is pure conjecture, supposition and narrative construction towards the creation of an apparently plausible explanation. Before building and decorating a tree that can stand on its own, first ensure it is well rooted and can support the upper structure you want to create.
Seems like it would be much easier and cheaper just to take quercetin, zinc, vit C, and get some sunshine daily. And never drink crap from the tap or get jabbed! I have not had a cold for 15 years or flu for 20 years and have not been jabbed since 2009.
From my perspective, which is not that of a scientist, this science is full of holes that shouldn't have been left there by the supervising FDA scientists. Was this done intentionally? It seems that the FDA's friends at Pfizer and Moderna can simply say it wasn't their stuff or it was expired and walk away from any responsibility or need to answer any further questions in the public mind. On the positive side, I think the professional scientists involved have been challenged by some high school kids, and they had better answer that challenge and prove them and their methods and theories were wrong, which i don't think they can do. So expect ad hominem attacks. Also, were the FDA scientists may have been trying to say something through the high schoolers without saying it themselves? Heh heh.:-)
Am I reading this correctly - we are now making mRNA vaccines in high school labs? Is the next stop junior high kids making them in their basements?
There was a TV series called Biohacker with a project Homo Deus. https://thecinemaholic.com/biohackers-ending-explained/
This all sounds a lot like that.
Only if the high school is within proximity of a BSL-1 lab.
Still...a bit worrisome.
Several years ago (preCOVID) there was report of a high school science project doing CRISPR.
DIY biology may be the end of us!
And the New Labs would actually Benefit Us
This all seems like a bit of a red herring to divert attention from the fact that the medical industrial complex continues to perform horrendous experimentation on humanity.
OK. So was the paper valid, partially valid, invalid? Does the study need to be redone by others to see what they find? I think that would be a good approach.
All studies should be replicable by others.
That's the benefit of Pharma studies with 29,000 subjects- nobody can afford to replicate them. cf "Jupiter Study" for rosuvastatin with the conclusion that every adult male is at risk so should be treated.
Read McKernan's substack (referenced above) on this to get a fuller picture of its validity.
Replication of studies is supposed to be the norm to test validity of the results.
It depends on what your analysis goal is. As the comment by the good Dr.😉
The pcDNA3.1(+) is an old skool mammalian expression vector Maria. Its dual purpose becuase it can be used to express a coding sequence in mammalian cells, or transcribe + stranded RNA (which could be a RNA riboprobe, or an mRNA if the RNA is capped and polyadenylated). It was sold by Invitrogen who were later gobbled up by ThermoFisher. The orientation of the multiple cloning site (MCS: a short sequence with useful restriction enzyme sites where you can clone the coding region for the thing you want to express under the control of the strong CMV promoter/enhancer) comes in + or - orientations. In this case it just means that the order of the sites is reversed in - versus +.
https://assets.thermofisher.com/TFS-Assets/vectors/pcDNA3.1plus_mcs.pdf
The SV40 early promoter/origin or replication has 2 main functions - to drive expression of the neomycin/kanamycin resistance gene to enable selection of transfected DNA (usually integrated as a stable cell line) in cells with neomycin (G418) - as in the Pfizer vector. SV40 ori also allows amplification/maintenance of the plasmid in mammalian cells that carry SV40 large T antigen, such as HEK293T - which gives higher expression of whatever you clone in the MCS.
https://assets.thermofisher.com/TFS-Assets/vectors/pcDNA3.1plus_map.pdf
The ampicillin gene has a bacterial promoter only and is used for selection in E.coli, and replicate the plasmid to high copy number using the pMB1 E.coli origin of replication.
They would have grown the plasmid in E.coli, made a plasmid DNA prep, then linearized the plasmid with an enzyme site that cuts downstream of the sequence they wanted to make mRNA from - then transcribed the + strand as RNA using T7 polymerase.
Thanks! So that is what MCS stands for.
So the AmpR promoter tells us it was grown in E. coli
I am bothered by the fact that the in vitro transcription step wasn't stated. I assume this was done as you describe.
pMB1 origin (there are others) is needed to replicate the plasmid in E.coli. The Amp promoter/resistance gene (encoding beta-lactamase enzyme) enables selection and maintenance of the plasmid.
They refer in the method to in vitro translation of the plasmid but don't explain how they did it and if they used modified nucleotide or not. I suspect they were give the mRNA and just did the lipid encapsulation using the Cayman kit.
My suspicion as well
Thanks very much for mentioning my work on this substandard US Military FDA Psyop.
The juicier discoveries will be in part 2, but you will see enough hints in part 1.
https://geoffpain.substack.com/p/us-fda-censors-school-student-measurement
The " bastard class " are still guilty and still continue to cover up , divert , or look away ......all the overwhelming evidence by all the experts just highlights the most important fact .....there was NEVER any informed consent and the public were conspired against .
I applaud all those trying to prove that point .....🤷♂️🙏
Interesting Maria, that you found the 2023 FDA Laboratory award involving 3 named scientists, including Tony T Wang. He has a Phd in microbiology and works at FDA dept of CBER (Center for Biologics Evaluation and Research) which is located at the FDA’s White Oak Campus in Silver Spring, MD, just outside of Washington, DC.
https://www.fda.gov/vaccines-blood-biologics/science-research-biologics/understanding-interplay-between-host-immunity-and-viral-factors-rational-design-vaccines-against
The 3 students (Tyler Wang, Alex Kim & Kevin Kim) from Centerville High School Virginia, won a Science Fair special award in March 2024 for their project (3rd place Certificate $50 split) under "Association for Diagnostics and Laboratory Medicine (ADLM)"
https://www.fcps.edu/2024-science-fair-special-awards
Coincidentally the FDA CBER lab location is also where the 3 high school students were supervised by FDA researchers (presumably in 2023).
I found that Tony Wang is one of 6 authors in an article published in Dec. 2024.
https://www.researchgate.net/publication/386876232_Combined_mutations_in_nonstructural_protein_14_envelope_and_membrane_proteins_mitigate_the_neuropathogenicity_of_SARS-CoV-2_Omicron_BA1_in_K18-hACE2_mice
Apart from the "observations" mentioned in this article, I wonder too if Tony Wang at the FDA is possibly related to the student Tyler Wang.
Thanks for sharing this
Thanks for the update on this article Dr. Jessica Rose 🌹 this is very confusing to me. My first assumption on reading the first post of this was that they got samples of Moderna and Pfizer and they found in it high DNA contamination this muddies the waters of what this paper really does show in my view ?
Regarding why FDA researchers might be developing a Live Attenuated Vaccine delivered intranasally:
1- Intranasal delivery will lead to mucosal immunity, whereas intramuscular injection leads to very poor mucosal immunity.
2- Omicron is nature's attenuated vaccine but it was first identified in Nov 2021 and had yet to demonstrate how benign it was. The Liu and colleagues paper was received May 10 2022. So at the time, it would've made sense to develop a LAV.
Akiko Iwasaki was trying to get funding for her own intranasal vaccine.
It looks like the LAV is developed through genetic engineering, and not through traditional methods (serial passage through eggs, getting viruses from non-human species, etc.). Operation Warp Speed was very biased towards high-technology approaches and away from low-tech approaches like Sinovac, traditional LAVs, etc. The high-tech approach tends to have patentable components which can generate money for big pharma.
https://www.nature.com/articles/s41467-022-34571-4#:~:text=In%20summary%2C%20our%20studies%20demonstrate,sterilizing%20immunity%20in%20the%20lung.
Thanks for sharing this Update Jessica 🙏